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The purpose of The
Visible Spectra of Grape Juice lab was to use a spectrophotometer to
calculate the wavelengths of a solution with different concentrations.
Transmittance and absorbance of the solution would be given by the
spectrophotometer, and then plotted on an x-y graph to find the slope of
either the transmittance or absorbance. In this lab, we used of
concentrated grape juice and water with known ratios. 11 test tubes were
set up, each with a ratio of juice to water, differing each time by 1 mL.
When these ratios have been plotted consistently, a linear graph is
created with a constant slope. Therefore, when given an unknown
concentration of grape juice and water, the transmittance and absorption
can be obtained from the machine and then placed into the constant slope.
The x and y factors will be the ratios of water and grape juice.
It can be predicted that, from the information we have gathered about the
nature of the wavelengths determined by the spectrophotometer, the slopes
of both transmittance and absorbance will be linear. Thus, it is likely
that an unknown concentration x of grape juice and water will be able to
have a specific transmittance/absorbance by matching both factors to the
generated slope.
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